UJI RESISTENSI BAKTERI Escherichia coli DARI SUMBER AIR BAKU DI KARAWANG TERHADAP ANTIBIOTIK SIPROFLOKSASIN

  • Fuji Ayu Diniarti Universitas Singaperbangsa Karawang
  • Ahsanal Kasasiah Universitas Singaperbangsa Karawang
  • Indah Laily Hilmi Universitas Singaperbangsa Karawang
Keywords: Antibiotics, Ciprofloxacin, Escherichia coli, Raw Water

Abstract

Infectious diseases are included in the ten most common diseases in Indonesia. The disease can be overcome by the use of antimicrobials, one of which is antibiotics. Antibiotics are drugs used to treat infections caused by bacteria. Excessive use of antibiotics without clear indications can have an impact on the occurrence of bacterial resistance to these antibiotics. One of the bacteria that is resistant to antibiotics is Escherichia coli. This study aims to determine the sensitivity of Escherichia coli isolated from raw water sources in Karawang to the antibiotic ciprofloxacin. Samples were obtained from irrigation water which is the source of raw water for one branch of Perumdam in Karawang. The samples were tested using Lactose Broth (LB) media, then confirmed using Eosin Methylene Blue (EMBA) media to confirm the presence of Escherichia coli bacteria equipped with Gram stain. Bacterial resistance test was carried out using Mueller Hinton Agar (MHA) media with disc diffusion method. The results showed that the average diameter of the clear zone formed around the paper disc was 30.16 mm. The isolates showed intermediate results against the antibiotic ciprofloxacin.

Downloads

Download data is not yet available.

References

Aslan, A., Cole, Z., Bhattacharya, A., & Oyibo, O. (2018). Presence of antibiotic-resistant Escherichia coli in wastewater treatment plant effluents utilized as water reuse for irrigation. Water, 10, 805.

Astuti, D., & Arfania, M. (2018). Analisis Penggunaan Antibiotika Dengan Metoda Atc/Ddd Di Rumah Sakit Swasta Kab Karawang, Pharma Xplore : Jurnal Ilmiah Farmasi, 3(2), pp. 194–202. doi: 10.36805/farmasi.v3i2.467.

Balasa, G., Levengood, E.S., Battistelli, J.M., & Franklin, R.B. (2021). Diversity of Multidrug-Resistant Bacteria in an Urbanized River: A Case Study of the Potential Risks from Combined Sewage Overflows. Water 13, 2122. https://doi.org/10.3390/w13152122

Pusdik. (2018). Beberapa Media yang Biasa Digunakan dalam Analisa Coliform, diperoleh melalui situs
http://www.pusdik.kkp.go.id/elearning/index.php/modul/read/181219-014912beberapa-c-media-c-yang-c-biasa-c-digunakan-c-dalam-c-analisa-c-coliform. Diunduh pada tanggal 8 April 2022.

CLSI. (2020). Performance Standards for Antimicrobial Susceptibility Testing. 30th ed. CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute. 39-49; 201.

Dhafin A. (2017). Analisis Cemaran Bakteri Coliform Escherichia coli pada Bubur Bayi Home Industry di Kota Malang dengan Metode TPC dan MPN. Skripsi. Program Studi Farmasi. Fakultas Kedokteran dan Ilmu-Ilmu Kesehatan. Universitas Islam Negeri Maulana Malik Ibrahim. Malang. 56-58.

Dirga., Khairunnisa, S.M., Akhmad, A.D., Setyawan, I.A., & Pratama, A. (2021). Evaluasi Penggunaan Antibiotik pada Pasien Rawat Inap di Bangsal Penyakit Dalam RSUD. Dr. H. Abdul Moeloek Provinsi Lampung. Jurnal Kefarmasian Indonesia, 11(1), 65–75. https://doi.org/10.22435/jki.v11i1.3570

Harijani, N., Rahadi, U.S.E., & Nazar, D.S. (2013). Isolasi Escherichia coli pada Daging yang Diperoleh dari Beberapa Pasar Tradisional di Surabaya Selatan,Veterinaria Medika, 29(2). 1–17.

Kemenkes. (2018). Laporan Provinsi Jawa Barat, Riskesdas 2018, Lembaga Penerbit Badan Penelitian dan Pengembangan Kesehatan.

Lupo, A., Coyne, S., & Berendonk, T. U. (2012). Origin and Evolution of Antibiotic Resistence: The Common Mechanisms of Emergence and Spread in Water Bodies. Frontiers in Microbiology, 3. doi: 10.3389/fmicb.2012.00018.

Perbup. (2020). Peraturan Bupati Karawang Nomor 77 Tahun 2020 Tentang Rencana Aksi Daerah Penyediaan Air Minum Dan Penyehatan Lingkungan Kabupaten Karawang Tahun 2020.

Permenkes. (2015). Peraturan Menteri Kesehatan Republik Indonesia Nomor 8 Tahun 2015 Tentang Program Pengendalian Resistensi Antimikroba di Rumah Sakit.

Putri, A, R. (2017). Resistensi Eschericia coli dari Isolat Daging Ayam Broiler terhadap Tetrasiklin di Peternakan Kecamatan Sumbersari Kabupaten Jember. Skripsi. Jember: Universitas Jember. 1–99.

Rachmad, B., Saputri,W., Yandi, A.S., Setiawan, A., & Mulyono. (2017). Isolasi dan Identifikasi Gen Resisten Ciprofloxacin pada Isolat Escherichia coli MDR Ciprofloxacin dari Penderita ISK di RSUDAM Provinsi Lampung. JK Unila, 1(3).

Rahmaniar, R.P., Widhowati, D., & Hidayah, N. (2019). Sensitivitas Antimikroba Terhadap Bakteri Escherichia coli yang Diisolasi dari Udang di Pasar Keputran Surabaya. Jurnal Kajian Veteriner. 7(2), 93-100.

Setiabudy, R., & Gunawan, S.G., (2016). Farmakologi dan Terapi, hal. 725, FKUI, Jakarta.

Sharma, D., Patel, R.P., Zaidi, S.T.R., Sarker, M.M.R., Lean, Q.Y., Ming, L.C. (2017). Interplay of the Quality of Ciprofloxacin and Antibiotic Resistance in Developing Countries. Front. Pharmacol. 8:546. doi: 10.3389/fphar.2017.00546

Sholih, M. G., Sudarjat, H. & Saula, L. S. (2019). Gambaran Penggunaan Antibiotik Berdasarkan Metode ATC/DDD dan DU 90% di Salah Satu Puskesmas Karawang. HealthGambaran Penggunaan Antibiotik Berdasarkan Metode ATC/DDD dan DU 90% di Salah Satu Puskesmas Karawang. Health.

Suciari, L.K., Mastra, N., W. C. D. (2018). Perbedaan Zona Hambat Pertumbuhan Staphylococcus Aureus pada Berbagai Konsentrasi Rebusan Daun Salam (Syzygium Polyanthum) Secara In Vitro. Meditory : The Journal of Medical Laboratory. 5(2), 92–100. https://doi.org/10.33992/m.v5i2.138

Syafriana, V., Hamida, F., Sukamto, A.R., & Aliya, L.S. (2020). Resistensi Escherichia coli dari Air Danau ISTN Jakarta Terhadap Antibiotik Amoksisilin, Tetrasiklin, Kloramfenikol, dan Siprofloksasin. Saintech Farma: Jurnal Ilmu Kefarmasian, 13(2), 92–98.
https://ejournal.istn.ac.id/index.php/saintechfarma/article/view/717.

Tankeshwar, A. (2021). What does Susectible, Intermediate, and Resistant Mean?, diperoleh melalui situs https://microbeonline.com/interpretation-susceptibility-testing-susceptible-intermediate-resistant-mean/. Diunduh pada tanggal 18 April 2022.

Zakki, G. I. (2015). Skripsi Pengetahuan dan Perilaku Preventif Terhadap Bakteri E. coli pada Masyarakat Kecamatan Gondomanan di Kota Yogyakarta.
Published
2022-09-30
How to Cite
Diniarti, F., Kasasiah, A., & Hilmi, I. (2022). UJI RESISTENSI BAKTERI Escherichia coli DARI SUMBER AIR BAKU DI KARAWANG TERHADAP ANTIBIOTIK SIPROFLOKSASIN. Jurnal Riset Kefarmasian Indonesia, 4(3), 414-429. https://doi.org/10.33759/jrki.v4i3.281
Views Statistic
  • Abstract 205
  • PDF 144