POTENSI EKSTRAK N-HEKSAN, DIKLOROMETANA, ETIL ASETAT, DAN ETANOL 70% JAMUR DEWA (Agaricus blazei Murill) TERHADAP SEL MCF-7

  • Misgiati - Misgiati Akademi Analis Farmasi dan Makanan Putra Indonesia Malang
Keywords: MCF-7 cells, Extraction levels, God mushroom

Abstract

God mushroom is a functional food. It has pharmacological activity. This content has activity as an anticancer. The purpose of this study was to conduct a preliminary test to obtain an active ingredient capable of having anticancer activity on MCF-7 cells. The stages of the research are stratified extraction with several solvents. Extraction using maceration. The resulting extract was identified and tested for the anticancer activity of MCF-7 cells. The test uses the TLC method. The stationary phase with silica GF254, the mobile phase is n-hexane: ethyl acetate. Anticancer activity test using the MTT method. The results of the identification of each extract contained terpenoid secondary metabolites. Anticancer activity of MCF-7 cells with IC50 extract of n-hexane 15.0923 µg/ml, dichloromethane extract 17.4213 µg/ml, ethyl acetate extract 10.9132µg/ml with strong cytotoxicity criteria, while for ethanol extract IC50 675, 1236 µg/ml.

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Published
2022-01-27
How to Cite
Misgiati, M. (2022). POTENSI EKSTRAK N-HEKSAN, DIKLOROMETANA, ETIL ASETAT, DAN ETANOL 70% JAMUR DEWA (Agaricus blazei Murill) TERHADAP SEL MCF-7. Jurnal Riset Kefarmasian Indonesia, 4(1), 11-23. https://doi.org/10.33759/jrki.v4i1.231
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